Permanent Superhumps in V1974 Cyg

We present results of 32 nights of CCD photometry of V1974 Cygni, from the years 1994 and 1995. We verify the presence of two distinct periodicities in the light curve: 0.0812585 day~1.95 hours and 0.0849767 d~2.04 hr. We establish that the shorter periodicity is the orbital period of the underlying binary system. The longer period oscillates with an average value of |dot(P)| ~ 3x10^(7)--typical to permanent superhumps. The two periods obey the linear relation between the orbital and superhump periods that holds among members of the SU Ursae Majoris class of dwarf novae. A third periodicity of 0.083204 d~2.00 hr appeared in 1994 but not in 1995. It may be related to the recently discovered anti-superhump phenomenon. These results suggest a linkage between the classical nova V1974 Cyg and the SU UMa stars, and indicate the existence of an accretion disk and permanent superhumps in the system no later than 30 months after the nova outburst. From the precessing disk model of the superhump phenomenon we estimate that the mass ratio in the binary system is between 2.2 and 3.6. Combined with previous results this implies a white dwarf mass of 0.75-1.07 M sun.Comment: 11 pages, 10 eps. figures, Latex, accepted for publication in MNRA

PUMA amplifies necroptosis signaling by activating cytosolic DNA sensors.

Necroptosis, a form of regulated necrotic cell death, is governed by RIP1/RIP3-mediated activation of MLKL. However, the signaling process leading to necroptotic death remains to be elucidated. In this study, we found that PUMA, a proapoptotic BH3-only Bcl-2 family member, is transcriptionally activated in an RIP3/MLKL-dependent manner following induction of necroptosis. The induction of PUMA, which is mediated by autocrine TNF-α and enhanced NF-κB activity, contributes to necroptotic death in RIP3-expressing cells with caspases inhibited. On induction, PUMA promotes the cytosolic release of mitochondrial DNA and activation of the DNA sensors DAI/Zbp1 and STING, leading to enhanced RIP3 and MLKL phosphorylation in a positive feedback loop. Furthermore, deletion of PUMA partially rescues necroptosis-mediated developmental defects in FADD-deficient embryos. Collectively, our results reveal a signal amplification mechanism mediated by PUMA and cytosolic DNA sensors that is involved in TNF-driven necroptotic death in vitro and in vivo

Engineering liver

Interest in “engineering liver” arises from multiple communities: therapeutic replacement; mechanistic models of human processes; and drug safety and efficacy studies. An explosion of micro- and nanofabrication, biomaterials, microfluidic, and other technologies potentially affords unprecedented opportunity to create microphysiological models of the human liver, but engineering design principles for how to deploy these tools effectively toward specific applications, including how to define the essential constraints of any given application (available sources of cells, acceptable cost, and user-friendliness), are still emerging. Arguably less appreciated is the parallel growth in computational systems biology approaches toward these same problems—particularly in parsing complex disease processes from clinical material, building models of response networks, and in how to interpret the growing compendium of data on drug efficacy and toxicology in patient populations. Here, we provide insight into how the complementary paths of engineering liver—experimental and computational—are beginning to interplay toward greater illumination of human disease states and technologies for drug development.National Institutes of Health (U.S.) (UH2TR000496)National Institutes of Health (U.S.) (R01-EB 010246)National Institutes of Health (U.S.) (R01-ES015241)National Institutes of Health (U.S.) (P30-ES002109

Results of the Australian geodetic VLBI experiment

The 250-2500 km baseline vectors between radio telescopes located at Tidbinbilla (DSS43) near Canberra, Parkes, Fleurs (X3) near Sydney, Hobart and Alice Springs were determined from radio interferometric observations of extragalactic sources. The observations were made during two 24-hour sessions on 26 April and 3 May 1982, and one 12-hour night-time session on 28 April 1982. The 275 km Tidbinbilla - Parkes baseline was measured with an accuracy of plus or minus 6 cm. The remaining baselines were measured with accuracies ranging from 15 cm to 6 m. The higher accuracies were achieved for the better instrumented sites of Tidbinbilla, Parkes and Fleurs. The data reduction technique and results of the experiment are discussed

Dry shear aligning: a simple and versatile method to smooth and align the surfaces of carbon nanotube thin films

Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.We show that the application of lateral shear force on a randomly oriented thin film of carbon nanotubes, in the dry state, causes significant reordering of the nanotubes at the film surface. This new technique of dry shear aligning is applicable to carbon nanotube thin films produced by many of the established methods

Single Proton Knock-Out Reactions from 24,25,26F

The cross sections of the single proton knock-out reactions from 24F, 25F, and 26F on a 12C target were measured at energies of about 50 MeV/nucleon. Ground state populations of 6.6+-.9 mb, 3.8+-0.6 mb for the reactions 12C(24F,23O) and 12C(25F,24O) were extracted, respectively. The data were compared to calculations based on the many-body shell model and the eikonal theory. In the reaction 12C(26F,25O) the particle instability of 25O was confirmed

Differential modulation of cellular death and survival pathways by conjugated bile acids

BACKGROUND: The liver-derived McNtcp.24 cells transport bile acids and show distinctive responses to the two classes of conjugated bile acids. Whereas taurine-conjugated bile acids are non-toxic, glycine-conjugated bile acids efficiently induce apoptosis. The aim of this study was to determine if the differential sensitivity is limited to cells that normally transport bile acids and if bile acid binding proteins could reduce bile acid-mediated apoptosis. The apical sodium/bile acid co-transporter (asbt) was expressed in Chinese hamster ovary (CHO) cells to establish active bile acid transport in a non-liver-derived cell model (CHO.asbt). A high-affinity bile acid binder was expressed in McNtcp.24 cells. RESULTS: The tolerance of McNtcp.24 cells to taurine-conjugated bile acids was associated with the stimulation of phosphatidylinositol 3-kinase (PI3K) activity. Treatment of CHO.asbt cells with taurine- and glycine-conjugated bile acids resulted in apoptosis. Unlike in McNtcp.24 cells, PI3K activity was not increased in CHO.asbt cells treated with taurine-conjugated bile acids. High level expression of a bile acid binder did not attenuate bile acid-induced cytotoxicity in McNtcp.24 cells. CONCLUSION: The data suggest that McNtcp.24 cells possess a mechanism that can elaborate distinctive responses to the different classes of bile acids. Additionally, activation of a signaling pathway involving PI3K appears to be the dominant mechanism responsible for the tolerance of McNtcp.24 cells to taurine-conjugated bile acids

Semiconductor resonator solitons above band gap

We show experimentally the existence of bright and dark spatial solitons in semiconductor resonators for excitation above the band gap energy. These solitons can be switched on, both spontaneously and with address pulses, without the thermal delay found for solitons below the band gap which is unfavorable for applications. The differences between soliton properties above and below gap energy are discussed.Comment: 4 pages, 7 figure

Comparison of Simulator Wear Measured by Gravimetric vs Optical Surface Methods for Two Million Cycles

Understanding wear mechanisms are key for better implants Critical to the success of the simulation Small amount of metal wear can have catastrophic effects in the patient such as heavy metal poisoning or deterioration of the bone/implant interface leading to implant failure Difficult to measure in heavy hard-on-hard implants (metal-on-metal or ceramic-on-ceramic) May have only fractions of a milligram of wear on a 200 g component At the limit of detection of even high-end balances when the component is 200 g and the change in weight is on the order of 0.000 1 grams Here we compare the standard gravimetric wear estimate with A non-contact 3D optical profiling method at each weighing stop A coordinate measuring machine (CMM) at the beginning and end of the ru

Lipids promote survival, proliferation, and maintenance of differentiation of rat liver sinusoidal endothelial cells in vitro

Primary rat liver sinusoidal endothelial cells (LSEC) are difficult to maintain in a differentiated state in culture for scientific studies or technological applications. Relatively little is known about molecular regulatory processes that affect LSEC differentiation because of this inability to maintain cellular viability and proper phenotypic characteristics for extended times in vitro, given that LSEC typically undergo death and detachment around 48–72 h even when treated with VEGF. We demonstrate that particular lipid supplements added to serum-free, VEGF-containing medium increase primary rat liver LSEC viability and maintain differentiation. Addition of a defined lipid combination, or even oleic acid (OA) alone, promotes LSEC survival beyond 72 h and proliferation to confluency. Moreover, assessment of LSEC cultures for endocytic function, CD32b surface expression, and exhibition of fenestrae showed that these differentiation characteristics were maintained when lipids were included in the medium. With respect to the underlying regulatory pathways, we found lipid supplement-enhanced phosphatidylinositol 3-kinase and MAPK signaling to be critical for ensuring LSEC function in a temporally dependent manner. Inhibition of Akt activity before 72 h prevents growth of SEC, whereas MEK inhibition past 72 h prevents survival and proliferation. Our findings indicate that OA and lipids modulate Akt/PKB signaling early in culture to mediate survival, followed by a switch to a dependence on ERK signaling pathways to maintain viability and induce proliferation after 72 h. We conclude that free fatty acids can support maintenance of liver LSEC cultures in vitro; key regulatory pathways involved include early Akt signaling followed by ERK signaling.National Science Foundation (U.S.) (Grant EFRI-0735997)National Institutes of Health (U.S.) (Grant R01 GM069668